Mercadé A, Sánchez A, Folch J M
Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Anim Biotechnol. 2007;18(1):23-35. doi: 10.1080/10495390601091073.
CDP-diacylglycerol synthase (CDS) catalyzes the conversion of phosphatidic acid to CDP-diacylglycerol, an important precursor for the synthesis of phosphatidylinositol, phosphatidylglycerol, and cardiolipin. We amplified and sequenced 2,053 bp of the pig CDS1 mRNA. The structure of the pig CDS1 gene was determined, being very similar to that of the human, rat, and mouse genes with respect size and organization of the 13 exons. In addition, we identified three polymorphic positions in exons 10 and 11. One of them, the A/C1006, was genotyped in samples belonging to Iberian, Landrace, Large White, Pietrain, and Meishan pig breeds. Expression of this gene was also analyzed by real-time polymerase chain reaction (PCR) in different tissues showing a high CDS1 expression in testis. Moreover, a 1240-bp fragment of the pig CDS2 mRNA was amplified and sequenced. Finally, the CDS1 and CDS2 genes were physically mapped to porcine chromosomes 8 and 17, respectively, by using the INRA, University of Minnesota porcine Radiation Hybrid panel (IMpRH).
CDP - 二酰基甘油合酶（CDS）催化磷脂酸转化为CDP - 二酰基甘油，后者是合成磷脂酰肌醇、磷脂酰甘油和心磷脂的重要前体。我们扩增并测序了猪CDS1 mRNA的2053 bp。确定了猪CDS1基因的结构，其在13个外显子的大小和组织方面与人类、大鼠和小鼠的基因非常相似。此外，我们在第10和11外显子中鉴定出三个多态性位点。其中一个，即A/C1006，在属于伊比利亚、长白、大白、皮特兰和梅山猪品种的样本中进行了基因分型。还通过实时聚合酶链反应（PCR）分析了该基因在不同组织中的表达，结果显示睾丸中CDS1表达较高。此外，扩增并测序了猪CDS2 mRNA的1240 bp片段。最后，利用法国国家农业研究院、明尼苏达大学猪辐射杂种细胞系（IMpRH）将CDS1和CDS2基因分别物理定位到猪的8号和17号染色体上。
