精子存活：与不育男性年龄相关的精子DNA完整性的关系。

Sperm survival: relationship to age-related sperm DNA integrity in infertile men.

作者信息

Moskovtsev S I, Willis J, White J, Mullen J B M

机构信息

Andrology Laboratory, Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada.

出版信息

Arch Androl. 2007 Jan-Feb;53(1):29-32. doi: 10.1080/01485010600908330.

DOI:10.1080/01485010600908330

PMID:17364462

Abstract

The purpose of our study was to evaluate the effect of age and sperm DNA integrity on sperm survival. Semen samples from fifty six unselected patients undergoing infertility evaluation were assessed in terms of standard semen parameters, DNA integrity, and sperm survival after 6-24 h of incubation. Prolonged incubation of density gradient selected sperm adversely effects sperm survival in older patients and patients with extensive sperm DNA damage. Immediate preparation of such samples prior to use for assisted reproductive technology (ART) may overcome the negative effect of extended incubation.

摘要

我们研究的目的是评估年龄和精子DNA完整性对精子存活的影响。对56例接受不孕症评估的未经过筛选的患者的精液样本进行了标准精液参数、DNA完整性以及孵育6 - 24小时后精子存活情况的评估。密度梯度筛选后的精子长时间孵育对老年患者和精子DNA广泛损伤的患者的精子存活有不利影响。在用于辅助生殖技术（ART）之前立即制备此类样本可能会克服延长孵育的负面影响。