Chen Shi-Hua, Guo Shan Li, Wang Zeng Lan, Zhao Ji Qiang, Zhao Yan Xiu, Zhang Hui
Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, People's Republic of China.
DNA Seq. 2007 Feb;18(1):61-7. doi: 10.1080/10425170601108555.
Halophytes can grow under a high salinity condition. Similar to glycophytes, their salt-tolerance possesses a high genetic complexity. There are many morphological and physiological studies on halophytes but very little information is at molecular level why they are salt-tolerant. Limonium sinense is a salt-secreting halophyte and can excretes salts by multi-cellular glands. Here, we report the library construction and sequence analysis of a cDNA library made from leaf tissue of L. sinenes. Among those 1082 expressed sequence tag (EST) obtained, 684 unique genes were identified: 429 showed homology to previously identified genes, 255 matched to uncharacterized genes. Compared with other EST databases, some characteristic features such as abundance genes in related to cytoskeleton and intracellular traffic, membrane transporting were observed, which may be specific to halophytes.
盐生植物能够在高盐度条件下生长。与甜土植物相似，它们的耐盐性具有很高的遗传复杂性。目前对盐生植物已有许多形态学和生理学方面的研究，但在分子水平上，关于它们为何耐盐的信息却非常少。中华补血草是一种泌盐盐生植物，能够通过多细胞腺体分泌盐分。在此，我们报道了由中华补血草叶片组织构建的cDNA文库及其序列分析结果。在所获得的1082个表达序列标签（EST）中，鉴定出684个独特基因：其中429个与先前鉴定的基因具有同源性，255个与未鉴定的基因相匹配。与其他EST数据库相比，观察到一些特征，如与细胞骨架、细胞内运输、膜转运相关的丰度基因，这些可能是盐生植物所特有的。
