海胆（光棘球海胆）骨形态发生蛋白的克隆与测序

Cloning, sequencing of bone morphogenetic protein from sea urchin, Hemicentrotus pulcherrimus.

作者信息

Kurogi Yumi, Ohta Kazumasa, Nakazawa Tohru, Tosuji Hiroaki

机构信息

Department of Chemistry and Bioscience, Faculty of Science, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.

出版信息

DNA Seq. 2007 Apr;18(2):112-9. doi: 10.1080/10425170601017186.

DOI:10.1080/10425170601017186

PMID:17364822

Abstract

A cDNA coding for bone morphogenetic protein (BMP) homolog of the sea urchin, Hemicentrotus pulcherrimus, was isolated from mid-gastrula using reverse transcription-polymerase chain reaction (RT-PCR) technique. The 2314 nucleotide sequence contains a 1383 open reading frame corresponding to a translation product of 461 amino acids. Comparison of the nucleotide and deduced amino acid sequence with BMP isolated from Strongylocentrotus purpuratus (SpBMP5-7; accession No. Z48313) shows a high degree of conservation. HpBMP seems to belong to the 60A subgroup as a result. A mRNA coding H. pulcherrimus BMP (HpBMP) was not detected in the unfertilized egg, but it was detected from blastula to prism stages.

摘要

利用逆转录-聚合酶链反应（RT-PCR）技术，从中间原肠胚中分离出了编码海胆（Hemicentrotus pulcherrimus）骨形态发生蛋白（BMP）同源物的cDNA。该2314个核苷酸序列包含一个1383个核苷酸的开放阅读框，对应于一个461个氨基酸的翻译产物。将该核苷酸和推导的氨基酸序列与从紫球海胆（Strongylocentrotus purpuratus）中分离出的BMP（SpBMP5-7；登录号Z48313）进行比较，结果显示出高度的保守性。因此，HpBMP似乎属于60A亚组。在未受精卵中未检测到编码海胆BMP（HpBMP）的mRNA，但在囊胚期到棱柱期均检测到了该mRNA。