Kurogi Yumi, Ohta Kazumasa, Nakazawa Tohru, Tosuji Hiroaki
Department of Chemistry and Bioscience, Faculty of Science, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
DNA Seq. 2007 Apr;18(2):112-9. doi: 10.1080/10425170601017186.
A cDNA coding for bone morphogenetic protein (BMP) homolog of the sea urchin, Hemicentrotus pulcherrimus, was isolated from mid-gastrula using reverse transcription-polymerase chain reaction (RT-PCR) technique. The 2314 nucleotide sequence contains a 1383 open reading frame corresponding to a translation product of 461 amino acids. Comparison of the nucleotide and deduced amino acid sequence with BMP isolated from Strongylocentrotus purpuratus (SpBMP5-7; accession No. Z48313) shows a high degree of conservation. HpBMP seems to belong to the 60A subgroup as a result. A mRNA coding H. pulcherrimus BMP (HpBMP) was not detected in the unfertilized egg, but it was detected from blastula to prism stages.
利用逆转录-聚合酶链反应（RT-PCR）技术，从中间原肠胚中分离出了编码海胆（Hemicentrotus pulcherrimus）骨形态发生蛋白（BMP）同源物的cDNA。该2314个核苷酸序列包含一个1383个核苷酸的开放阅读框，对应于一个461个氨基酸的翻译产物。将该核苷酸和推导的氨基酸序列与从紫球海胆（Strongylocentrotus purpuratus）中分离出的BMP（SpBMP5-7；登录号Z48313）进行比较，结果显示出高度的保守性。因此，HpBMP似乎属于60A亚组。在未受精卵中未检测到编码海胆BMP（HpBMP）的mRNA，但在囊胚期到棱柱期均检测到了该mRNA。
