Zhu Hong-Liang, Zhu Ben-Zhong, Shao Yi, Lin Xi-Jin, Wang Xiao-Guang, Gao Hong-Yan, Xie Yun-Hong, Li Ying-Cong, Luo Yun-Bo
Laboratory of Fruit Biology, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu, Beijing 100083, People's Republic of China.
DNA Seq. 2007 Apr;18(2):131-7. doi: 10.1080/10425170601060921.
Recently, ETHYLENE OVERPRODUCER 1 (ETO1) had been cloned and identified as a negative post-transcriptional regulator in the ethylene biosynthesis in Arabidopsis. However, little was known about the role of ETO1 in other species, especially in tomato, which was an ideal model for studying the biosynthesis of ethylene during tomato fruit ripening. In this study, a tomato ETHYLENE OVERPRODUCER 1-LIKE 1 (LeEOL1) was cloned. The LeEOL1 cDNA was 3,515 bp long and carried an ORF that putatively encoded a polypeptide of 886 amino acids with a predicted molecular mass of 95 kDa. It shared 74% identity in amino acid sequence with Arabidopsis EOL1 and had one BTB (Broad-complex, Tramtrack, Bric-à-brac) domain and two TPR (tetratricopeptide repeat) domains, which were also conserved domains in AtEOL1. RT-PCR analysis of the temporal expression of LeEOL1 showed that its transcript decreased companied with increase of ethylene production in tomato ripening. The level of LeEOL1 transcripts in wild type tomato fruit at mature green stage did not distinctively change when treated with exogenous ethylene.
最近，乙烯过量产生蛋白1（ETO1）已被克隆，并被鉴定为拟南芥乙烯生物合成中的负转录后调节因子。然而，关于ETO1在其他物种中的作用知之甚少，尤其是在番茄中，番茄是研究番茄果实成熟过程中乙烯生物合成的理想模型。在本研究中，克隆了一个番茄乙烯过量产生蛋白1类似物1（LeEOL1）。LeEOL1 cDNA长3515 bp，带有一个开放阅读框，推测编码一个由886个氨基酸组成的多肽，预测分子量为95 kDa。其氨基酸序列与拟南芥EOL1的同一性为74%，并具有一个BTB（Broad-complex、Tramtrack、Bric-à-brac）结构域和两个TPR（四肽重复）结构域，这些也是AtEOL1中的保守结构域。对LeEOL实时定量PCR分析表明，在番茄成熟过程中，其转录本随着乙烯产量的增加而减少。用外源乙烯处理时，野生型番茄果实成熟绿熟期的LeEOL1转录本水平没有明显变化。 1转录本的时间表达分析表明，其转录本随着番茄成熟过程中乙烯产量的增加而减少。野生型番茄果实成熟绿熟期的LeEOL1转录本水平在用外源乙烯处理时没有明显变化。
