抗氧化剂/亲电试剂反应元件基序

The antioxidant/electrophile response element motif.

作者信息

Nerland Donald E

机构信息

Department of Pharmacology & Toxicology, Health Sciences Center, University of Louisville, Louisville, KY, USA.

出版信息

Drug Metab Rev. 2007;39(1):235-48. doi: 10.1080/03602530601125000.

DOI:10.1080/03602530601125000

PMID:17364885

Abstract

Cells exposed to oxidative stress or electrophilic xenobiotics respond by transcriptionally up-regulating a battery of genes that contain a cis-acting element in their promoter region known as the antioxidant/electrophile response element (ARE). Mutational analysis of the promoter regions of ARE-containing genes led to the creation of two different models for the ARE; a core ARE (cARE: RTGACnnnGC) and an extended ARE (eARE: TMAnnRTGAYnnnGCAwwww). Using bioinformatic software we have aligned the promoter regions of several ARE-containing genes to produce two position-specific probability matrices that independently describe the cARE and eARE. These matrices can also be used to quantitatively assess putative AREs.

摘要

暴露于氧化应激或亲电子异生物质的细胞会通过转录上调一系列基因做出反应，这些基因在其启动子区域含有一个顺式作用元件，称为抗氧化/亲电子反应元件（ARE）。对含ARE基因的启动子区域进行突变分析，产生了两种不同的ARE模型；核心ARE（cARE：RTGACnnnGC）和扩展ARE（eARE：TMAnnRTGAYnnnGCAwwww）。我们使用生物信息学软件对几个含ARE基因的启动子区域进行比对，以生成两个独立描述cARE和eARE的位置特异性概率矩阵。这些矩阵也可用于定量评估假定的ARE。