Garrel Catherine, Ceballos-Picot Irene, Germain Guy, Al-Gubory Kaïs H
Laboratoire de Biologie du Stress Oxydant, Département de Biologie Intégrée, Centre Hospitalier Universitaire de Grenoble, France.
Free Radic Res. 2007 Mar;41(3):251-9. doi: 10.1080/10715760601067493.
Oxidative stress-induced antioxidant adaptive response would be particularly important to cells in high reactive oxygen species (ROS) environments. We aimed to determine the dynamic adaptive response of antioxidant enzymatic systems in sheep corpus luteum (CL) during PGF2alpha-induced luteal cell death. Activities of superoxide dismutase (SOD1 and SOD2), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GSR), and in situ DNA fragmentation were determined in CL at day 10 of the estrous cycle (0 h) and at 12, 24 or 48 h after PGF2alpha injection. A decrease in plasma progesterone concentration was first observed at 6 h after treatment (P < 0.05). Apoptotic cells were rarely observed in the CL at 0 h (less than 0.7%), and their incidence increased (P < 0.01) by 12 h post-PGF2alpha (11.7%) and remained thereafter elevated through 48 h. Activities of SOD1, SOD2, GPX and GSR were not changed at any time points after PGF2alpha treatment. CAT activity increased at 12 h (P < 0.01) and at 24 h (P < 0.05) after PGF2alpha treatment as compared to that at 0 h. These findings demonstrate that PGF2alpha induce luteal cell death without depressing the activity of antioxidant enzymes. It is suggested that transient increase in CAT activity is an adaptive response of the CL to oxidative stress induced by PGF2alpha.
氧化应激诱导的抗氧化适应性反应对处于高活性氧（ROS）环境中的细胞尤为重要。我们旨在确定在前列腺素F2α（PGF2α）诱导的黄体细胞死亡过程中，绵羊黄体（CL）抗氧化酶系统的动态适应性反应。在发情周期第10天（0小时）以及PGF2α注射后12、24或48小时，测定黄体中超氧化物歧化酶（SOD1和SOD2）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽还原酶（GSR）的活性以及原位DNA片段化情况。治疗后6小时首次观察到血浆孕酮浓度下降（P < 0.05）。在0小时时，黄体中很少观察到凋亡细胞（少于0.7%），PGF2α注射后12小时其发生率增加（P < 0.01）至11.7%，此后直至48小时一直保持升高。PGF2α处理后任何时间点，SOD1、SOD2、GPX和GSR的活性均未改变。与0小时相比，PGF2α处理后12小时（P < 0.01）和24小时（P < 0.05）CAT活性增加。这些发现表明，PGF2α诱导黄体细胞死亡而不抑制抗氧化酶的活性。提示CAT活性的短暂增加是黄体对PGF2α诱导的氧化应激的一种适应性反应。
