Taechowisan Thongchai, Tuntiwachwuttikul Pittaya, Lu Chunhua, Shen Yuemao, Lumyong Saisamorn, Taylor Walter C
Department of Microbiology, Faculty of Science, Silpakorn University, Nakorn, Pathom, Thailand.
Immunol Invest. 2007;36(2):203-11. doi: 10.1080/08820130600992115.
This research was undertaken to test the in vitro anti-inflammatory action of 5,7,4'-trimethoxy-4-phenylcoumarin and 5,7-dimethoxy-4-phenylcoumarin produced by Streptomyces aureofaciens CMUAc130. The effects of the two coumarins were investigated on the formation of NO, PGE2, and TNF-alpha and also on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells. The data obtained were consistent with the modulation of iNOS enzyme expression. A similar effect was also observed when LPS-induced PGE2 release and COX-2 expression were tested. The inhibitory effects were shown in concentration-dependent manners. The 5,7,4'-Trimethoxy-4-phenylcoumarin and 5,7-dimethoxy-4-phenylcoumarin also mildly but significantly reduced the formation of TNF-alpha.
本研究旨在测试金色链霉菌CMUAc130产生的5,7,4'-三甲氧基-4-苯基香豆素和5,7-二甲氧基-4-苯基香豆素的体外抗炎作用。研究了这两种香豆素对脂多糖（LPS）诱导的小鼠巨噬细胞RAW 264.7细胞中一氧化氮（NO）、前列腺素E2（PGE2）和肿瘤坏死因子-α（TNF-α）形成的影响，以及对诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的影响。获得的数据与iNOS酶表达的调节一致。在测试LPS诱导的PGE2释放和COX-2表达时也观察到了类似的效果。抑制作用呈浓度依赖性。5,7,4'-三甲氧基-4-苯基香豆素和5,7-二甲氧基-4-苯基香豆素也轻微但显著地减少了TNF-α的形成。
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