Coffinier Yannick, Szunerits Sabine, Jama Charafeddine, Desmet Rémi, Melnyk Oleg, Marcus Bernadette, Gengembre Léon, Payen Edmond, Delabouglise Didier, Boukherroub Rabah
Institut de Recherche Interdisciplinaire (IRI, FRE2963) and Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN), Cité Scientifique, Avenue Poincaré - BP. 60069, 59652 Villeneuve d'Ascq, France.
Langmuir. 2007 Apr 10;23(8):4494-7. doi: 10.1021/la063440y. Epub 2007 Mar 17.
This paper reports on the formation and characterization of semicarbazide termination on aminated boron-doped diamond (BDD) surfaces, and further preparation of peptide microarray through site-specific alpha-oxo semicarbazone ligation. Hydrogen-terminated BDD electrodes were first aminated using NH3 plasma treatment and then reacted with triphosgene and Fmoc-protected hydrazine to yield a protected semicarbazide termination. Subsequent deprotection and chemical reaction with glyoxylyl peptides led to the covalent immobilization of the peptides on the surface through site-specific ligation. The resulting surfaces were characterized using X-ray photoelectron spectroscopy (XPS) and fluorescence measurements.
本文报道了氨基化硼掺杂金刚石（BDD）表面氨基脲封端的形成与表征，以及通过位点特异性α-氧代半卡巴腙连接进一步制备肽微阵列。首先使用NH3等离子体处理对氢封端的BDD电极进行氨基化，然后使其与三光气和Fmoc保护的肼反应，得到受保护的氨基脲封端。随后的脱保护以及与乙醛酰基肽的化学反应导致肽通过位点特异性连接共价固定在表面上。使用X射线光电子能谱（XPS）和荧光测量对所得表面进行表征。
