Institut de Recherche Interdisciplinaire (IRI, USR 3078), Université Lille Nord de France, Parc de la Haute Borne, 50 Avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.
Anal Chem. 2010 Oct 1;82(19):8203-10. doi: 10.1021/ac1016387.
This paper describes the label-free detection of carbohydrate-lectin interactions. The sensor consists of a boron-doped diamond (BDD) electrode terminated with alkynyl surface groups, which have been functionalized via the CuACC (copper(I)-catalyzed azide-alkyne cycloaddition) "click" reaction with carbohydrate analogues bearing an azido-terminating arm. In this work, electrochemical impedance spectroscopy (EIS) was used as an effective technique to probe the specific interactions of the surface-bound carbohydrates with their complementary lectin partners, and the response was found to be dependent on the relative density of sugar units immobilized on the BDD surface. A BDD interface with 60% surface-bound mannose showed a detection limit of ∼5 ± 0.5 nM for Lens culinaris lectin, with an affinity constant of K(A) = (2.63 ± 0.5) × 10(6) M(-1).
本文描述了无标记的糖-凝集素相互作用检测。该传感器由硼掺杂金刚石（BDD）电极组成，其末端带有炔基表面基团，通过带有叠氮末端臂的糖类似物与 CuACC（铜（I）-催化的叠氮-炔环加成反应）“点击”反应进行功能化。在这项工作中，电化学阻抗谱（EIS）被用作探测表面结合的碳水化合物与互补凝集素伴侣特异性相互作用的有效技术，并且发现响应取决于固定在 BDD 表面上的糖单位的相对密度。具有 60%表面结合甘露糖的 BDD 界面对扁豆凝集素的检测限约为 5±0.5 nM，亲和常数 K(A)为（2.63±0.5）×10(6) M(-1)。
