Rojo-Bezares Beatriz, Sáenz Yolanda, Navarro Laura, Zarazaga Myriam, Ruiz-Larrea Fernanda, Torres Carmen
Departamento de Agricultura y Alimentación, Area de Bioqímica y Biología Molecular, Universidad de La Rioja, Madre de Dios 51, 26006 Logroño, Spain.
Food Microbiol. 2007 Aug;24(5):482-91. doi: 10.1016/j.fm.2006.09.003. Epub 2006 Oct 31.
Detection and characterization of bacteriocin production by Lactobacillus plantarum strain J23, recovered from a grape must sample in Spain, have been carried out. Bacteriocin activity was degraded by proteolytic enzymes (trypsin, alfa-chymotrypsin, papaine, protease, proteinase K and acid proteases), and it was stable at high temperatures (121 degrees C, 20min), in a wide range of pH (1-12), and after treatment with organic solvents. L. plantarum J23 showed antimicrobial activity against Oenococcus oeni, and a range of Lactobacillus and Pediococcus species. Bacteriocin production was detected in liquid media only when J23 was cocultivated with some inducing bacteria, and induction took place when intact cells or 55 degrees C heated cells of the inducer were cocultivated with J23, but not with their autoclaved cells. Bacteriocin activity of J23 was not induced by high initial J23 inocula, and it was detected in cocultures during the exponential phase. The presence of ethanol or acidic pH in the media reduced bacteriocin production in the cocultures of J23 with the inducing bacteria. The presence of plantaricin-related plnEF and plnJ genes was detected by PCR and sequencing. Nevertheless, negative results were obtained for plnA, plnK, plNC8, plS and plW genes.
对从西班牙一份葡萄汁样品中分离得到的植物乳杆菌J23菌株产生细菌素的情况进行了检测和特性分析。细菌素活性可被蛋白水解酶（胰蛋白酶、α-胰凝乳蛋白酶、木瓜蛋白酶、蛋白酶、蛋白酶K和酸性蛋白酶）降解，在高温（121℃，20分钟）、广泛的pH范围（1 - 12）以及用有机溶剂处理后均保持稳定。植物乳杆菌J23对酒酒球菌以及一系列乳酸杆菌和片球菌具有抗菌活性。仅当J23与一些诱导菌共培养时，在液体培养基中才检测到细菌素的产生，当诱导菌的完整细胞或经55℃加热的细胞与J23共培养时会发生诱导作用，但与它们的高压灭菌细胞共培养时则不会。J23的高初始接种量不会诱导细菌素活性，且在指数生长期的共培养物中可检测到细菌素活性。培养基中乙醇的存在或酸性pH会降低J23与诱导菌共培养时的细菌素产量。通过PCR和测序检测到了与植物乳杆菌素相关的plnEF和plnJ基因。然而，plnA、plnK、plnNC8、plnS和plnW基因的检测结果为阴性。
