Perez-Martinez Pablo, Perez-Jimenez Francisco, Ordovas Jose M, Bellido Cecilia, Moreno Juan A, Gomez Purificación, Marin Carmen, Fernandez de la Puebla Rafael Angel, Paniagua Juan Antonio, Lopez-Miranda Jose
Lipids and Atherosclerosis Research Unit, Reina Sofia University Hospital, Avda Menendez Pidal, s/n. 14004 Cordoba, Spain.
Nutr Metab Cardiovasc Dis. 2007 Mar;17(3):224-9. doi: 10.1016/j.numecd.2005.11.010. Epub 2006 Mar 20.
Our goal was to determine whether the presence of the -516C/T polymorphism in the APOB gene promoter modifies the lipid response to changes in the amount and quality of dietary fat. We studied 97 young healthy volunteers (70 males and 27 females), 62 homozygotes for the -516C allele (C/C) (47 males and 15 females), 34 heterozygotes for the -516T allele (C/T) (22 males and 12 females) and one male homozygote for the -516T allele (T/T). Subjects consumed three different diets in successive 4-week dietary periods. During the first 28 days, all subjects consumed a saturated fatty acid (SFA)-rich diet (38% fat and 20% SFA). Then, using a randomized crossover design, subjects were assigned a carbohydrate (CHO)-rich diet (30% fat and 55% carbohydrate) or a monounsaturated fatty acid (MUFA)-rich diet (38% fat and 22% MUFA). At the end of each dietary period, plasma concentrations of triacylglycerols and of total, LDL, and HDL cholesterol were measured. No differences in plasma lipid and apolipoprotein response were found after changes in dietary fat intake in relation to the -516C/T polymorphism in our study population. In conclusion, our data suggest that the APOB -516C/T polymorphism has no effect on the lipid profile after changes in dietary fat intake in a healthy population.
我们的目标是确定载脂蛋白B（APOB）基因启动子中-516C/T多态性的存在是否会改变脂质对膳食脂肪数量和质量变化的反应。我们研究了97名年轻健康志愿者（70名男性和27名女性），其中62名是-516C等位基因纯合子（C/C）（47名男性和15名女性），34名是-516T等位基因杂合子（C/T）（22名男性和12名女性），还有一名男性是-516T等位基因纯合子（T/T）。受试者在连续的4周饮食期内食用三种不同的饮食。在最初的28天里，所有受试者食用富含饱和脂肪酸（SFA）的饮食（38%脂肪和20% SFA）。然后，采用随机交叉设计，为受试者分配富含碳水化合物（CHO）的饮食（30%脂肪和55%碳水化合物）或富含单不饱和脂肪酸（MUFA）的饮食（38%脂肪和22% MUFA）。在每个饮食期结束时，测量血浆中甘油三酯以及总胆固醇、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇的浓度。在我们的研究人群中，就-516C/T多态性而言，饮食脂肪摄入量改变后，血浆脂质和载脂蛋白反应未发现差异。总之，我们的数据表明，在健康人群中，饮食脂肪摄入量改变后，APOB -516C/T多态性对脂质谱没有影响。
