[胆固醇酯转运蛋白TaqIB基因多态性对健康青年高碳水化合物/低脂饮食诱导的血脂比值变化的影响]

[Effects of cholesterol ester transfer protein taqIB polymorphism on changes of serum lipid ratios induced by high-carbohydrate/low-fat diet in healthy youth].

作者信息

Du Juan, Fang Ding-Zhi, Gong Ren-Rong, Tang Hui, Huang Xin, Li Rong-Hui

机构信息

Department of Biochemistry and Molecular Biology Laboratory, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Jul;40(4):684-8.

PMID:19764573

Abstract

OBJECTIVE

To investigate the effects of cholesterol ester transfer protein gene (CETP) TaqIB polymorphism on serum lipid ratios and their responses to high-carbohydrate/low-fat (HC/LF) diet in healthy youth.

METHODS

After on regular diet for 7 days, 56 health youth [(22.89+/-1.80) years] were given HC/LF diet for 6 days. The regular diet contained 54% carbohydrate, 15% protein, and 31% fat of the total energy. The LF/HC diet contained 70% carbohydrate, 15% protein, and 15% fat of the total energy. The serum lipids were measured on the 1st, the 8th and the 14th day. The ratios of TG/HDL-C, log (TG/HDL-C), TC/HDL-C, and LDL-C/HDL-C were calculated. The polymorphism of CETP was analyzed by PCR-RFLP method.

RESULTS

No significant difference was found of the lipid ratios at baseline in subjects with different genotypes in both genders. After the regular diet, TC/HDL-C was significantly lower in males carrying the B2 allele than B1B1 homozygotes (P<0.05). After HC/LF diet, no significant difference was found of the lipid ratios between the subjects with different genotypes in the whole study population or in males and females separately. Compared with those before HC/LF diet, subjects with B1B1 genotype experienced significant changes of the four lipid ratios after HC/LF diet (P<0.05), while the B2 carriers had only significant decrease of TC/HDL-C and LDL-C/HDL-C (P<0.05). No significant difference of the changes of the four lipid ratios before and after HC/LF diet was found between the subjects with different genotypes. When gender was taken into account, significant increases of TG/HDL-C and log (TG/HDL-C) were found in females with B1B1 genotype (P<0.05). All the subjects experienced significant decrease of TC/HDL-C and LDL-C/HDL-C regardless of gender and genotype (P<0.05). As for the changes of lipid ratios before and after HC/LF diet, difference of only TC/HDL-C was found between males with B1B1 and male carriers of B2 (P<0.05).

CONCLUSION

Polymorphism of CETP TaqIB may influence the response of TC/HDL-C to HC/LF diet in males and the increases of TG/HDL-C and log (TG/HDL-C) induced by HC/LF diet in females.

摘要

目的

探讨胆固醇酯转运蛋白基因（CETP）TaqIB多态性对健康青年血清脂质比值及其对高碳水化合物/低脂（HC/LF）饮食反应的影响。

方法

56名健康青年[（22.89±1.80）岁]在常规饮食7天后，给予HC/LF饮食6天。常规饮食中碳水化合物占总能量的54%、蛋白质占15%、脂肪占31%。LF/HC饮食中碳水化合物占总能量的70%、蛋白质占15%、脂肪占15%。在第1天、第8天和第14天测量血清脂质。计算甘油三酯/高密度脂蛋白胆固醇（TG/HDL-C）、log（TG/HDL-C）、总胆固醇/高密度脂蛋白胆固醇（TC/HDL-C）和低密度脂蛋白胆固醇/高密度脂蛋白胆固醇（LDL-C/HDL-C）的比值。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析CETP的多态性。

结果

不同基因型的男女受试者在基线时脂质比值无显著差异。常规饮食后，携带B2等位基因的男性的TC/HDL-C显著低于B1B1纯合子（P<0.05）。HC/LF饮食后，整个研究人群或分别在男性和女性中，不同基因型受试者的脂质比值无显著差异。与HC/LF饮食前相比，B1B1基因型受试者在HC/LF饮食后四种脂质比值有显著变化（P<0.05），而B2携带者只有TC/HDL-C和LDL-C/HDL-C显著降低（P<0.05）。不同基因型受试者在HC/LF饮食前后四种脂质比值变化无显著差异。考虑性别因素时，B1B1基因型女性的TG/HDL-C和log（TG/HDL-C）显著升高（P<0.05）。无论性别和基因型，所有受试者的TC/HDL-C和LDL-C/HDL-C均显著降低（P<0.05）。至于HC/LF饮食前后脂质比值的变化，B1B1基因型男性与B2基因携带者男性之间仅TC/HDL-C存在差异（P<0.05）。