用于膜蛋白稳定化和结晶的去污剂。

Detergents for the stabilization and crystallization of membrane proteins.

作者信息

Privé Gilbert G

机构信息

Division of Cancer Genomics and Proteomics, Ontario Cancer Institute, Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, Ontario, Canada.

出版信息

Methods. 2007 Apr;41(4):388-97. doi: 10.1016/j.ymeth.2007.01.007.

DOI:10.1016/j.ymeth.2007.01.007

PMID:17367711

Abstract

The use of detergents for the structural study of membrane proteins is discussed with an emphasis on practical issues relating to membrane solubilization, protein aggregation, detergent purity and detergent quantitation. Detergents are useful reagents as mimics of lipid bilayers because of their self-assembling properties, but as a result, they have complex properties in solution. It can be difficult to maintain a solubilized membrane protein in a native conformational state, and the non-specific aggregation of detergent-solubilized proteins is a common problem. Empirical "stability screens" can be helpful in choosing which detergents, and which detergent concentrations, may be optimal for a given system.

摘要

本文讨论了洗涤剂在膜蛋白结构研究中的应用，重点关注与膜溶解、蛋白质聚集、洗涤剂纯度和洗涤剂定量相关的实际问题。洗涤剂由于其自组装特性，可作为脂质双层的模拟物，是有用的试剂，但因此它们在溶液中具有复杂的性质。将溶解的膜蛋白维持在天然构象状态可能很困难，洗涤剂溶解的蛋白质的非特异性聚集是一个常见问题。经验性的“稳定性筛选”有助于选择对于给定系统可能是最佳的洗涤剂及其浓度。