说明性章节：选择合适的洗涤剂。

Explanatory chapter: choosing the right detergent.

作者信息

Linke Dirk

机构信息

Department Protein Evolution, Max-Planck-Institute for Developmental Biology, Tübingen, Germany.

出版信息

Methods Enzymol. 2014;541:141-8. doi: 10.1016/B978-0-12-420119-4.00011-2.

DOI:10.1016/B978-0-12-420119-4.00011-2

PMID:24674068

Abstract

If working with membrane proteins, practically all biochemical techniques can be used as for soluble proteins, the important point being that detergents must be added to keep the membrane proteins in solution. This protocol is meant to help in choosing the right detergent for a given application.

摘要

如果处理膜蛋白，实际上所有用于可溶性蛋白的生化技术都可以使用，关键在于必须添加去污剂以使膜蛋白保持在溶液中。本方案旨在帮助为特定应用选择合适的去污剂。