用支原体去除剂处理的受支原体污染和未受污染的人成纤维细胞培养物中溶酶体水解酶活性测量的比较。
Comparison of the measurement of lysosomal hydrolase activity in mycoplasma-contaminated and non-contaminated human fibroblast cultures treated with mycoplasma removal agent.
作者信息
Souza Fernanda Timm Seabra, Souza Sostruznik Luana, Casagrande Scolari Roberta, Maciel de Castro Karen Joana, Giugliani Roberto, Coelho Janice Carneiro
机构信息
Departamento de Bioquímica, ICBS, UFRGS, Porto Alegre, RS, Brazil.
出版信息
Clin Biochem. 2007 May;40(8):521-5. doi: 10.1016/j.clinbiochem.2007.01.016. Epub 2007 Feb 3.
OBJECTIVES
To investigate the effect of both mycoplasma contamination and of its remover (MRA), through human fibroblasts culture over the activity of some lysosomal hydrolases.
DESIGN AND METHODS
Activity was measured in contaminated fibroblasts before and after the addition of MRA. Results were compared with the enzymatic activity in control fibroblasts with and without MRA.
RESULTS
Only beta-glucosidase showed no significant alteration in the presence of either mycoplasma or MRA. Total hexosaminidase and beta-galactosidase underwent significant interference in the presence of the mycoplasma and the MRA. The % of hexosaminidase A and arylsulphatase A altered their activity only in the presence of MRA. Beta-glucuronidase changed its activity only in the presence of mycoplasma.
CONCLUSIONS
The fibroblast enzymes behaved differently in the presence of MRA and/or mycoplasma, demonstrating the sensitivity of these hydrolases. Our work suggests that mycoplasma and MRA alter the activity of some lysosomal hydrolases.
目的
通过人成纤维细胞培养，研究支原体污染及其去除剂（MRA）对某些溶酶体水解酶活性的影响。
设计与方法
在添加MRA之前和之后，测量受污染成纤维细胞中的活性。将结果与添加和未添加MRA的对照成纤维细胞中的酶活性进行比较。
结果
仅β-葡萄糖苷酶在存在支原体或MRA的情况下未显示出明显变化。总己糖胺酶和β-半乳糖苷酶在存在支原体和MRA的情况下受到显著干扰。己糖胺酶A和芳基硫酸酯酶A的百分比仅在存在MRA的情况下改变其活性。β-葡萄糖醛酸酶仅在存在支原体的情况下改变其活性。
结论
成纤维细胞酶在存在MRA和/或支原体的情况下表现不同，证明了这些水解酶的敏感性。我们的工作表明支原体和MRA会改变某些溶酶体水解酶的活性。
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