Fu Haiyan, Zeng Guangming, Zhong Hua, Yuan Xingzhong, Wang Wei, Huang Guohe, Li Jianbing
Department of Environmental Engineering, Xiamen University of Technology, Xiamen, China.
Colloids Surf B Biointerfaces. 2007 Aug 1;58(2):91-7. doi: 10.1016/j.colsurfb.2007.02.012. Epub 2007 Feb 20.
The effect of rhamnolipid produced by a Pseudomonas aeruginosa strain on the aerobic degradation of granular organic substrate from kitchen waste by the bacterium was studied and compared with that of two synthetic surfactants, SDS and Triton X-100. The adsorption of rhamnolipid on the substrate, the surfactant-interfered adhesion of bacteria on the substrate as well as physicochemical and microbial conditions of the substrate during degradation were investigated. The adsorption isotherm of rhamnolipid on the substrate fit Freundlich law and its interactions with the substrate and bacteria weakened the adsorption of the bacteria on the substrate. The two synthetic surfactants, however, did not have such microbial effects. During degradation, rhamnolipid slowed down water evaporation in the substrate and significantly strengthened the dispersion of organic matter into the substrate water phase. The number of cells in the rhamnolipid treatment was higher than that in control and the remaining organic matter content in the substrates also had faster decreasing. SEM examination showed the on-site degradation of the substrate organic matter without rhamnolipid and the transfer of the degradation site in the presence of rhamnolipid. The results indicated that interference of rhamnolipid in the substrate matrix plays a potential role, physicochemically or microbially, on the degradation of the granular organic substrate. SDS and Triton X-100 may have the above physicochemical effects, but not so significant.
研究了铜绿假单胞菌菌株产生的鼠李糖脂对该细菌好氧降解厨余颗粒有机底物的影响,并与两种合成表面活性剂十二烷基硫酸钠(SDS)和 Triton X - 100 进行了比较。研究了鼠李糖脂在底物上的吸附、表面活性剂干扰细菌在底物上的黏附以及降解过程中底物的物理化学和微生物条件。鼠李糖脂在底物上的吸附等温线符合 Freundlich 定律,其与底物和细菌的相互作用削弱了细菌在底物上的吸附。然而,这两种合成表面活性剂没有这种微生物效应。在降解过程中,鼠李糖脂减缓了底物中的水分蒸发,并显著增强了有机物在底物水相中的分散。鼠李糖脂处理中的细胞数量高于对照,底物中剩余有机物含量的下降也更快。扫描电子显微镜(SEM)检查显示,无鼠李糖脂时底物有机物的原位降解以及有鼠李糖脂时降解位点的转移。结果表明,鼠李糖脂对底物基质的干扰在物理化学或微生物方面对颗粒有机底物的降解具有潜在作用。SDS 和 Triton X - 100 可能具有上述物理化学效应,但不那么显著。