Yuan XingZhong, Ren FangYi, Zeng GuangMing, Zhong Hua, Fu HaiYan, Liu Jia, Xu XinMiao
College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
Appl Microbiol Biotechnol. 2007 Oct;76(5):1189-98. doi: 10.1007/s00253-007-1080-z. Epub 2007 Jul 19.
The adsorption behavior of five surfactants, cetyltrimethylammonium bromide (CTAB), Triton X-100, Tween 80, sodium dodecyl sulfate (SDS), and rhamnolipid, on a Pseudomonas aeruginosa strain and the effect of temperature and ionic strength (IS) on the adsorption were studied. The change of cell surface lypohydrophilic property caused by surfactant adsorption was also investigated. The results showed that the adsorption kinetics of the surfactants on the cell followed the second-order law. CTAB adsorption was the fastest one under the experimental conditions, and it took longest for SDS adsorption to equilibrate because of electric repulsion. The adsorption of Triton X-100 and Tween 80 was characterized by short equilibration time, and rhamnolipid adsorption reached equilibrium in about 90 min. The adsorption isotherms of all the surfactants on the bacterium fitted Freundlich equation well, but the adsorption capacity and mode were variations for the surfactants as indicated by k and n parameters in the equations. The adsorption mode for all the surfactants except SDS is probably hydrophilic interaction because the adsorption totally turned the cell surface to be more hydrophobic. Neither the temperature nor the IS had significant effect on CTAB adsorption, but higher IS significantly enhanced SDS adsorption and modestly strengthened adsorption of Triton X-100, Tween 80, and rhamnolipid. Higher temperature strengthened adsorption of SDS but weakened the adsorption of Triton X-100, Tween 80, and rhamnolipid.
研究了十六烷基三甲基溴化铵（CTAB）、Triton X-100、吐温80、十二烷基硫酸钠（SDS）和鼠李糖脂这五种表面活性剂在铜绿假单胞菌菌株上的吸附行为，以及温度和离子强度（IS）对吸附的影响。还研究了表面活性剂吸附引起的细胞表面亲疏水性变化。结果表明，表面活性剂在细胞上的吸附动力学符合二级定律。在实验条件下，CTAB的吸附最快，而SDS由于电排斥作用达到平衡所需时间最长。Triton X-100和吐温80的吸附特点是平衡时间短，鼠李糖脂吸附在约90分钟内达到平衡。所有表面活性剂在细菌上的吸附等温线都很好地拟合了Freundlich方程，但方程中的k和n参数表明，表面活性剂的吸附容量和模式存在差异。除SDS外，所有表面活性剂的吸附模式可能是亲水相互作用，因为吸附使细胞表面完全变得更疏水。温度和IS对CTAB的吸附均无显著影响，但较高的IS显著增强了SDS的吸附，并适度增强了Triton X-100、吐温80和鼠李糖脂的吸附。较高的温度增强了SDS的吸附，但减弱了Triton X-100、吐温80和鼠李糖脂的吸附。
