通过在小颗粒上进行反相高效液相色谱法分离C27、C28和C29甾醇。
Separation of C27, C28 and C29 sterols by reversed-phase high-performance liquid chromatography on small particles.
作者信息
Rees H H, Donnahey P L, Goodwin T W
出版信息
J Chromatogr. 1976 Jan 21;116(2):281-91. doi: 10.1016/s0021-9673(00)89899-4.
The application of reversed-phase high-performance liquid chromatography to the analytical- and preparative-scale separation of sterols has been evaluated. The capacity factors, k', for a number of compounds chromatographed on a muBondapak C18 (LESS THAN 10 MUM) COLUMN ARE PRESENTED. C27, C28 and C29 sterols and also sterols differing in degree of unsaturation could be readily separated as their acetates in this system. The present reversed-phase chromatographic method is apparently not as selective as silver nitrate-silica gel thin-layer chromatography for the position of unsaturation in the sterol molecule.
已经评估了反相高效液相色谱法在甾醇分析规模和制备规模分离中的应用。给出了在μBondapak C18（小于10μm）柱上色谱分析的多种化合物的容量因子k'。在该系统中，C27、C28和C29甾醇以及不饱和度不同的甾醇都可以很容易地以它们的乙酸酯形式分离。对于甾醇分子中不饱和度的位置，目前的反相色谱方法显然不如硝酸银-硅胶薄层色谱法有选择性。
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