Mishra Neeraj, Gupta Prem N, Mahor Sunil, Khatri Kapil, Goyal Amit K, Vyas Suresh P
Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences Dr Harisingh Gour Vishwavidyalaya, Sagar 470 003, India.
Indian J Exp Biol. 2007 Mar;45(3):237-41.
In the present study tetanus toxoid (TT) loaded liposomes and diphtheria toxoid (DT) loaded liposomes were prepared by reverse phase evaporation method and after combining these two vaccines the potential advantages were investigated. Prepared systems were characterized for the size, shape and entrapment efficiency. SDS-PAGE analysis of TT and DT was also performed. The selected liposomal formulations were administered subcutaneously to Balb/c mice and their immune responses were determined using ELISA after 15, 30, 45 days. After boosting the maximum immune response was observed after 45 days and was found to be 0.831 and 0.749 for TT loaded liposome and DT loaded liposomes respectively. When the mice were immunized subcutaneously with the physical mixture of TT loaded liposomes and DT loaded liposomes the immune response for the combination vaccine was found to be 1.44 and 0.741 for the TT and DT respectively. The result showed that the immune response of TT increased when it was combined with DT in liposomes. This confirms adjuvantcity of DT vis-a-vis immunogenicity. Thus, carrier mediated cocktail vaccination holds promise for clinical applications.
在本研究中，采用逆相蒸发法制备了破伤风类毒素（TT）负载脂质体和白喉类毒素（DT）负载脂质体，并在将这两种疫苗组合后研究了其潜在优势。对制备的体系进行了大小、形状和包封率表征。还对TT和DT进行了SDS-PAGE分析。将选定的脂质体制剂皮下注射给Balb/c小鼠，并在15、30、45天后使用ELISA测定其免疫反应。加强免疫后，在45天时观察到最大免疫反应，发现TT负载脂质体和DT负载脂质体的最大免疫反应分别为0.831和0.749。当用TT负载脂质体和DT负载脂质体的物理混合物皮下免疫小鼠时，发现联合疫苗中TT和DT的免疫反应分别为1.44和0.741。结果表明，当TT与脂质体中的DT联合时，其免疫反应增强。这证实了DT相对于免疫原性的佐剂作用。因此，载体介导的联合疫苗接种在临床应用中具有前景。
