Basu P S, Ali Masood, Chaturvedi S K
Indian Institute of Pulses Research (ICAR), Kanpur 208 024, India.
Indian J Exp Biol. 2007 Mar;45(3):261-7.
Eight chickpea advanced breeding lines (ABLs) and their parents were evaluated for osmotic adjustment (OA), leaf carbohydrates and gas exchange under dryland field . These (ABLs) were derived from crosses between CTS 60543 x Kaniva and Tyson x Kaniva. Mean leaf water potential (LWP) fell down from -1.00 MPa at pre-stress level to about -2.25 MPa during terminal stress. Relative water content (RWC) showed periodic changes with alternate decrease or increase at certain interval, which also influenced the values of OA (low or high) in number of genotypes e.g. Kaniva, CTS 60543, Tyson and M 75. Significant variation in OA ranging 0.45 to 0.88 MPa was observed at high level of stress at -2.5 MPa. However, none of the genotypes showed stability of OA over the period of stress. Leaf starch declined even at mild stress (LWP, -1.6 MPa) resulting in an increase in hexose sugars and activation state of sucrose-phosphate synthase (SPS) that led to accumulation of sucrose. Both photosynthesis (Pmax) and transpiration decreased concurrently in two chickpea lines M 129 and Tyson with increasing water stress. However, rate of decline in the photosynthesis slowed down even drought was further intensified. The observed periodic changes in OA, RWC and photosynthesis appeared to be associated with drought-induced changes in SPS and carbohydrates which modify water uptake of the leaves.
对8个鹰嘴豆高级育种系（ABLs）及其亲本进行了评估，以研究其在旱地田间条件下的渗透调节（OA）、叶片碳水化合物和气体交换情况。这些ABLs源自CTS 60543×卡尼瓦和泰森×卡尼瓦的杂交组合。在终端胁迫期间，平均叶片水势（LWP）从胁迫前水平的-1.00 MPa下降至约-2.25 MPa。相对含水量（RWC）呈现周期性变化，在特定间隔交替下降或上升，这也影响了多个基因型（如卡尼瓦、CTS 60543、泰森和M 75）的OA值（低或高）。在-2.5 MPa的高胁迫水平下，观察到OA的显著变化范围为0.45至0.88 MPa。然而，在胁迫期间没有一个基因型表现出OA的稳定性。即使在轻度胁迫（LWP，-1.6 MPa）下，叶片淀粉也会下降，导致己糖增加以及蔗糖磷酸合酶（SPS）的激活状态增加，从而导致蔗糖积累。随着水分胁迫增加，鹰嘴豆品系M 129和泰森的光合作用（Pmax）和蒸腾作用同时下降。然而，即使干旱进一步加剧，光合作用的下降速率也会减缓。观察到的OA、RWC和光合作用的周期性变化似乎与干旱诱导的SPS和碳水化合物变化有关，这些变化改变了叶片的水分吸收。
