Miyata Takaki, Saito Kanako, Nishizawa Yuji, Murayama Ayako, Masaoka Makoto, Ogawa Masaharu
Department of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya 466-8550, Japan.
Nagoya J Med Sci. 2005 Jun;67(3-4):65-70.
For the understanding of histogenetic events in the three-dimensional brain primordia, direct observation of progenitor cells and young neurons is required. Although slice culture, which is one of the tissue or organ culture methods, effectively preserves the in vivo microenvironment where normal developmental processes occur, conventional phase-contrast microscopic observation of brain slices fails to provide good visibility of single cells. However, a combination of slice culture with the use of fluorescent dyes and/or the introduction of fluorescent protein genes provides live, three-dimensional information on cytogenetic and histogenetic events at the individual cell level. Dynamic cellular behaviors can then be vividly captured without destroying tissue structures.
为了理解三维脑原基中的组织发生事件，需要直接观察祖细胞和年轻神经元。虽然切片培养作为组织或器官培养方法之一，能有效保留正常发育过程发生的体内微环境，但传统的脑切片相差显微镜观察无法清晰显示单个细胞。然而，将切片培养与荧光染料的使用和/或荧光蛋白基因的导入相结合，可在单个细胞水平上提供关于细胞发生和组织发生事件的实时三维信息。这样就能在不破坏组织结构的情况下生动地捕捉动态细胞行为。
