[Influential factor analysis and improvement of cultural method of oral mucosal epithelial cells].
作者信息
Wang Xiang, Gao Wen-xin, Wang Li, Wei Xiu-feng, Chen Ying-xin, Wang Xiao-feng
机构信息
Dept. of Oral Medicine, School of Stomatology, Jilin University, Changchun 130041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2007 Feb;25(1):79-82.
OBJECTIVE
To analyze influential factors of cultural method of human oral mucosal epithelial cells (hOMEC)and to establish a reliable cell culture system for hOMEC.
METHODS
Benzalkonium bromide and gentamicin sulfate were used to prevent microbial contamination. Separations of epithelium from underlying connective tissues with Dispase at different concentration were compared. Enzyme digestion was used to isolate cells and keratinocyte-serum free medium(K-SFM) was employed for primary culture and subculture of hOMEC.
RESULTS
Microbial contamination was under control. Separation of epithelium from underlying connective tissues with 0.40% Dispase was more complete than that of 0.25% Dispase. The cells grew fast and well in vitro.
CONCLUSION
The high successful culture of hOMEC and simplified procedures could be obtained with improvement of methods.
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