使用纳米结构电极研究细胞色素P450的电化学性质：直接电子转移和电催化作用

Electrochemical properties of cytochroms P450 using nanostructured electrodes: direct electron transfer and electro catalysis.

作者信息

Shumyantseva V V, Bulko T V, Rudakov Yu O, Kuznetsova G P, Samenkova N F, Lisitsa A V, Karuzina I I, Archakov A I

机构信息

Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya Street, 10, Moscow 119121, Russia.

出版信息

J Inorg Biochem. 2007 May;101(5):859-65. doi: 10.1016/j.jinorgbio.2007.01.015. Epub 2007 Feb 13.

DOI:10.1016/j.jinorgbio.2007.01.015

PMID:17376532

Abstract

The present study demonstrates direct electron transfer between cytochromes P450 2B4 (CYP2B4), P450 1A2 (CYP1A2), sterol 14alpha-demethylase (CYP51b1) on the one hand and screen-printed graphite electrodes, modified with gold nanoparticles and didodecyldimethylammonium bromide (DDAB) on the other. Electro detection of heme proteins was possible when 2-200 pmol P450/electrode were adsorbed on the surface of nanostructured electrochemical interfaces. Electron transfer, direct electrochemical reduction and interaction with P450 substrates (oxygen, benzphetamine, and lanosterol) and with P450 inhibitor (ketoconazole) were analyzed using cyclic voltammetry (CV), square wave voltammetry (SWV) differential pulse voltammetry (DPV), and amperometry.

摘要

本研究表明，细胞色素P450 2B4（CYP2B4）、P450 1A2（CYP1A2）、甾醇14α-脱甲基酶（CYP51b1）与另一方面用金纳米颗粒和二癸基二甲基溴化铵（DDAB）修饰的丝网印刷石墨电极之间存在直接电子转移。当2 - 200 pmol P450/电极吸附在纳米结构电化学界面表面时，对血红素蛋白进行电检测成为可能。使用循环伏安法（CV）、方波伏安法（SWV）、差分脉冲伏安法（DPV）和安培法分析了电子转移、直接电化学还原以及与P450底物（氧气、苄非他明和羊毛甾醇）和P450抑制剂（酮康唑）的相互作用。