Shumiantseva V V, Bulko T V, Kuznetsova G P, Samenkova N F, Bachman T, Schulze H, Schmid R, Usanov S A, Archakov A I
Biomed Khim. 2006 Jan-Feb;52(1):44-51.
A new approach for the electrochemical reduction of cytochromes P450 (P450s, CYPs) with electrodes chemically modified with appropriate substrates of P450s ("reverse" electrodes) has been proposed. The method is based on the analysis of cyclic voltammograms, square wave voltammograms, amperograms and determination of such electrochemical characteristics as catalytic current and redox potential. The sensitivity of the proposed method is 0.2-1 nmol P450/electrode. The differences of maximal current and potentials in square wave voltammograms and catalytic current in amperometric measurements are more sensitive and reliable. Planar regime of screen-printed electrodes permits to use 20-60 microl of electrolyte volume. We investigated P450 2B4--benzphetamine or P450scc--cholesterol enzyme - substrate pairs. Electrochemical parameters of electrodes with nonspecific P450 substrate were differed from electrodes with appropriate substrates.
提出了一种利用经细胞色素P450（P450s，CYPs）合适底物化学修饰的电极（“反向”电极）对细胞色素P450进行电化学还原的新方法。该方法基于对循环伏安图、方波伏安图、安培图的分析以及对催化电流和氧化还原电位等电化学特性的测定。所提方法的灵敏度为0.2 - 1 nmol P450/电极。方波伏安图中最大电流和电位的差异以及安培测量中的催化电流差异更为灵敏和可靠。丝网印刷电极的平面模式允许使用20 - 60微升的电解液体积。我们研究了P450 2B4 - 苄非他明或P450scc - 胆固醇酶 - 底物对。具有非特异性P450底物的电极的电化学参数与具有合适底物的电极不同。
