Young Douglas D, Deiters Alexander
North Carolina State University, Department of Chemistry, Campus Box 8204, Raleigh, NC 27695, USA.
Org Biomol Chem. 2007 Apr 7;5(7):999-1005. doi: 10.1039/b616410m. Epub 2006 Dec 20.
Photochemical regulation of biological processes offers a high level of control to study intracellular mechanisms with unprecedented spatial and temporal resolution. This report summarizes the advances made in recent years, focusing predominantly on the in vivo regulation of gene function using irradiation with UV light. The majority of the described applications entail the utilization of photocaging groups installed either on a small molecule modulator of biomolecular function or directly on a biological macromolecule itself.
生物过程的光化学调控提供了一种高度可控的手段，能够以前所未有的空间和时间分辨率研究细胞内机制。本报告总结了近年来取得的进展，主要聚焦于利用紫外线照射对基因功能进行体内调控。所描述的大多数应用都涉及使用安装在生物分子功能小分子调节剂上或直接安装在生物大分子本身上的光笼基团。
