利用聚合酶链反应方法检测土壤中的犬弓首蛔虫和猫弓首蛔虫卵。

Utilizing a polymerase chain reaction method for the detection of Toxocara canis and T. cati eggs in soil.

作者信息

Fogt-Wyrwas R, Jarosz W, Mizgajska-Wiktor H

机构信息

Department of Biology and Environmental Protection, University of Physical Education, Królowej Jadwigi 27/39, 61-871 Poznań, Poland.

出版信息

J Helminthol. 2007 Mar;81(1):75-8. doi: 10.1017/S0022149X07241872.

DOI:10.1017/S0022149X07241872

PMID:17381871

Abstract

A polymerase chain reaction (PCR) technique has been used for the differentiation of T. canis and T. cati eggs isolated from soil and previously identified from microscopical observations. The method, using specific primers for the identification of the two Toxocara species, was assessed in both the field and laboratory. Successful results were obtained when only a single or large numbers of eggs were recovered from 40 g soil samples. The method is sensitive, allows analysis of material independent of the stage of egg development and can be adapted for the recovery of other species of parasites from soil.

摘要

聚合酶链反应（PCR）技术已被用于区分从土壤中分离出的犬弓首蛔虫和猫弓首蛔虫卵，这些卵之前已通过显微镜观察鉴定。该方法使用特定引物来鉴定这两种弓首蛔虫，已在野外和实验室进行了评估。当从40克土壤样本中仅回收单个或大量虫卵时，获得了成功的结果。该方法灵敏，允许对与虫卵发育阶段无关的材料进行分析，并且可适用于从土壤中回收其他种类的寄生虫。