Wu Z, Nagano I, Xu D, Takahashi Y
Department of Parasitology, Gifu University School of Medicine, 40 Tsukasa, Gifu 500, Japan.
J Helminthol. 1997 Mar;71(1):77-8. doi: 10.1017/s0022149x00000845.
Primers for polymerase chain reaction to amplify genomic DNA of both Toxocara canis and T. cati were constructed by adapting cloning and sequencing random amplified polymorphic DNA. The primers are expected to detect eggs and/or larvae of T. canis and T. cati, both of which are known to cause toxocariasis in humans.
通过采用克隆和测序随机扩增多态性DNA的方法,构建了用于聚合酶链反应以扩增犬弓首蛔虫和猫弓首蛔虫基因组DNA的引物。预计这些引物可检测犬弓首蛔虫和猫弓首蛔虫的虫卵和/或幼虫,这两种寄生虫均已知会导致人类弓首蛔虫病。