真核生物tRNA（m7G46）甲基转移酶（Trm8-Trm82复合物）的RNA识别机制
RNA recognition mechanism of eukaryote tRNA (m7G46) methyltransferase (Trm8-Trm82 complex).
作者信息
Matsumoto Keisuke, Toyooka Takashi, Tomikawa Chie, Ochi Anna, Takano Yoshitaka, Takayanagi Naoyuki, Endo Yaeta, Hori Hiroyuki
机构信息
Department of Applied Chemistry, Faculty of Engineering, Ehime University, Bunkyo 3, Matsuyama 790-8577, Japan.
出版信息
FEBS Lett. 2007 Apr 17;581(8):1599-604. doi: 10.1016/j.febslet.2007.03.023. Epub 2007 Mar 16.
Yeast tRNA (m(7)G46) methyltransferase contains two protein subunits (Trm8 and Trm82). To address the RNA recognition mechanism of the Trm8-Trm82 complex, we investigated methyl acceptance activities of eight truncated yeast tRNA(Phe) transcripts. Both the D-stem and T-stem structures were required for efficient methyl-transfer. To clarify the role of the D-stem structure, we tested four mutant transcripts, in which tertiary base pairs were disrupted. The tertiary base pairs were important but not essential for the methyl-transfer to yeast tRNA(Phe) transcript, suggesting that these base pairs support the induced fit of the G46 base into the catalytic pocket.
酵母tRNA（m(7)G46）甲基转移酶包含两个蛋白质亚基（Trm8和Trm82）。为了探究Trm8-Trm82复合物的RNA识别机制，我们研究了8种截短的酵母tRNA(Phe)转录本的甲基接受活性。D茎和T茎结构对于高效甲基转移都是必需的。为了阐明D茎结构的作用，我们测试了4种突变转录本，其中三级碱基对被破坏。三级碱基对对于向酵母tRNA(Phe)转录本的甲基转移很重要，但不是必需的，这表明这些碱基对支持G46碱基诱导契合进入催化口袋。
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