Baltaziak Marek, Duraj Ewa, Koda Mariusz, Wincewicz Andrzej, Musiatowicz Marcin, Kanczuga-Koda Luiza, Szymanska Magdalena, Lesniewicz Tomasz, Musiatowicz Boguslaw
Department of Clinical and General Pathomorphology, Medical University of Bialystok, Waszyngtona 13, 15-269 Bialystok, Poland.
Ann N Y Acad Sci. 2006 Dec;1090:18-25. doi: 10.1196/annals.1378.002.
Disturbances in expression of apoptosis-associated proteins take part in the development and progression of many human malignancies. The aim of this study was the assessment of correlations among proteins involved in apoptosis-Bcl-xL, Bax, and p53-as well as relationships of these proteins with selected clinicopathological features in oral squamous cell carcinoma. Consequently, we examined by immunohistochemistry, using the avidin-biotin-peroxidase method, Bcl-xL, Bax, and p53 expression in 56 samples of primary oral squamous cell carcinoma and in 22 matched pairs of primary and metastatic tumors. The evaluation of immunostaining of Bcl-xL, Bax, and p53 was analyzed in 10 different tumor fields, and the mean percentage of tumor cells with positive staining was evaluated. The significance of the associations was determined using Spearman correlation analysis and the chi-square test. We found positive Bcl-xL, Bax, and p53 immunostaining in 44.6%, 28.6%, and 58.9% of the studied primary tumors and in 63.6%, 45.5%, and 72.7% of lymph node metastases, respectively. Analysis of associations among studied proteins revealed positive correlation between Bcl-xL and Bax in primary tumors (P<0.03, r=0.307). Statistically significant relationship between p53 expression in primary oral cancers and its expression in lymph node metastases (P<0.02) as well as increased expression of Bcl-xL, Bax, and p53 in metastatic sites compared with primary tumors could indicate an association of these proteins with oral cancer progression and development of metastases. Moreover, we suppose that knowledge about heterogeneity between primary and metastatic tumor might help to understand mechanisms of oral cancer progression.
凋亡相关蛋白表达的紊乱参与了许多人类恶性肿瘤的发生和发展。本研究的目的是评估凋亡相关蛋白——Bcl-xL、Bax和p53之间的相关性，以及这些蛋白与口腔鳞状细胞癌某些临床病理特征的关系。因此，我们采用抗生物素蛋白-生物素-过氧化物酶法，通过免疫组织化学检测了56例原发性口腔鳞状细胞癌样本以及22对匹配的原发性和转移性肿瘤样本中Bcl-xL、Bax和p53的表达。在10个不同的肿瘤区域分析Bcl-xL、Bax和p53免疫染色情况，并评估阳性染色肿瘤细胞的平均百分比。使用Spearman相关分析和卡方检验确定相关性的显著性。我们发现，在所研究的原发性肿瘤中，Bcl-xL、Bax和p53免疫染色阳性率分别为44.6%、28.6%和58.9%；在淋巴结转移瘤中，阳性率分别为63.6%、45.5%和72.7%。对所研究蛋白之间相关性的分析显示，原发性肿瘤中Bcl-xL和Bax呈正相关（P<0.03，r=0.307）。原发性口腔癌中p53表达与其在淋巴结转移中的表达之间存在统计学显著相关性（P<0.02），并且与原发性肿瘤相比，转移部位Bcl-xL、Bax和p53的表达增加，这可能表明这些蛋白与口腔癌进展和转移发生有关。此外，我们认为了解原发性肿瘤和转移性肿瘤之间的异质性可能有助于理解口腔癌进展的机制。
