Solenov E I, Katkova L E, Nesterov V V, Ivanova L N
Institute of Cytology and Genrtics of Russian Acad. Sci. Siberian branch, Novosibirsk Pr. Lavrentyeva 10, Russia.
Ross Fiziol Zh Im I M Sechenova. 2006 Nov;92(11):1358-64.
The role of AQP2,3 and intracellular calcium in vasopressin-induced increase in the water permeability of the basolateral cell membrane in microdissected rat kidney OMCD was studied. It was shown that increase in the water permeability of the basolateral membranes correlated with increase in the content of AQP2 and AQP3 in the membrane fraction isolated from outer kidney medulla. Preliminary loading of cells with BAPTA-AM which binds intracellular Ca2+ abolished the increase in the water permeability and prevented the rise of the AQP2 content in response to dDAVP. BAPTA was ineffective to block the enhancement of AQP2 content in membrane fraction in presence of dDAVP. These results suggest that the increase in intracellular calcium activity and the enhanced content of AQP2 in plasma membrane are important for the antidiuretic effect of dDAVP.
研究了水通道蛋白2、3（AQP2、3）及细胞内钙在抗利尿激素诱导的大鼠肾脏外髓集合管（OMCD）基底外侧细胞膜水通透性增加中的作用。结果表明，基底外侧膜水通透性的增加与从肾脏外髓分离的膜组分中AQP2和AQP3含量的增加相关。预先用结合细胞内Ca2+的BAPTA-AM处理细胞，可消除水通透性的增加，并阻止dDAVP诱导的AQP2含量升高。在存在dDAVP的情况下，BAPTA无法阻断膜组分中AQP2含量的增加。这些结果表明，细胞内钙活性的增加以及质膜中AQP2含量的增加对dDAVP的抗利尿作用至关重要。
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