[脱水和去氨加压素对肾集合管上皮细胞基底外侧膜水通透性的影响]

[Effect of dehydration and dDAVP on water permeability of basolateral membranes of epithelial cells in the kidney collecting tubules].

作者信息

Solenov E I, Baturina G S, Nesterov V V, Khodus G R, Ivanova L N

机构信息

Institute of Cytology and Genetics, Russian Acad. Sci., Siberian Branch, 10 Pr. Lavrentyeva, Novosibirsk, 630090, Russia.

出版信息

Ross Fiziol Zh Im I M Sechenova. 2002 Mar;88(3):387-95.

PMID:12013734

Abstract

Water permeability of the outer medullary collecting duct's (OMCD) basolateral membrane was determined in vitro in the tubules isolated from hyperhydrated or dehydrated Wistar rats. Oil was injected into the lumen to block apical membrane water permeability. OMCD fragments underwent a hypoosmic shock (600/300 mOsm) and epithelial cells volume increased ad recorded with a digital camera. The latter's rate was used to calculate apparent water permeability of the membrane (Pf). Treatment of the tubules with Hg2Cl2 suppressed the water permeability. Water deprivation and dDAVP induced an increase in the basolateral water permeability. The data obtained suggest that the water permeability of the OMCD basolateral membrane may be stimulated by vasopressin and water deprivation.

摘要

在从高水合或脱水的Wistar大鼠分离的肾小管中，体外测定了外髓集合管（OMCD）基底外侧膜的水通透性。向管腔内注入油以阻断顶端膜的水通透性。OMCD片段经历低渗休克（600/300 mOsm），上皮细胞体积增加，并用数码相机记录。用后者的速率来计算膜的表观水通透性（Pf）。用Hg2Cl2处理肾小管可抑制水通透性。禁水和去氨加压素可诱导基底外侧水通透性增加。所获得的数据表明，血管加压素和禁水可能会刺激OMCD基底外侧膜的水通透性。