Non-fusion proteins expressed in E. coli: detection by alpha-complementation (blue/white selection).
作者信息
Mensa-Wilmot K, Englund P T
机构信息
Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205.
出版信息
Nucleic Acids Res. 1992 Jan 11;20(1):143. doi: 10.1093/nar/20.1.143.
Abstract
摘要
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本文引用的文献
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cDNA encoding the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei.编码布氏锥虫糖基磷脂酰肌醇特异性磷脂酶C的互补DNA。
Proc Natl Acad Sci U S A. 1988 Dec;85(23):8914-8. doi: 10.1073/pnas.85.23.8914.
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Construction of mutant and chimeric genes using the polymerase chain reaction.利用聚合酶链反应构建突变基因和嵌合基因。
Nucleic Acids Res. 1989 Jan 25;17(2):723-33. doi: 10.1093/nar/17.2.723.
3
Expression plasmid containing the lambda PL promoter and cI857 repressor.含有λ PL 启动子和 cI857 阻遏蛋白的表达质粒。
Methods Enzymol. 1987;153:482-91. doi: 10.1016/0076-6879(87)53073-7.
4
An additional ribosome-binding site on mRNA of highly expressed genes and a bifunctional site on the colicin fragment of 16S rRNA from Escherichia coli: important determinants of the efficiency of translation-initiation.高表达基因mRNA上的一个额外核糖体结合位点以及来自大肠杆菌的16S rRNA的大肠杆菌素片段上的一个双功能位点：翻译起始效率的重要决定因素。
Nucleic Acids Res. 1989 Apr 25;17(8):2973-85. doi: 10.1093/nar/17.8.2973.
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Genomic organization, chromosomal localization, and developmentally regulated expression of the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei.布氏锥虫糖基磷脂酰肌醇特异性磷脂酶C的基因组组织、染色体定位及发育调控表达
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