[白细胞介素-1α对培养的人小梁网细胞吞噬功能的影响]
[Effect of interleukin-1alpha on the phagocytic function in cultured human trabecular meshwork cells].
作者信息
Li Xin-yu, Wang Chao-qiong, Xiang Nan, Zhou Xiong-wu
机构信息
Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Zhonghua Yan Ke Za Zhi. 2006 Nov;42(11):977-9.
OBJECTIVE
To investigate the effect of interleukin-1alpha (IL-1alpha) on the phagocytic function in cultured human trabecular meshwork (HTM) cells.
METHODS
HTM Cells were cultured and subcultured in vitro. The third to fifth passage of HTM cells were treated with different concentrations of IL-1alpha or vehicle in DMEM/F12 medium. 1000 of 0.85 microm microsphere/per cell were added to each well. The morphological changes of HTM cells were observed by an inverted phase-contrast microscope and electron microscope. The cellular phagocytic function of HTM cells was evaluated by counting the numbers of microsphere in 20 HTM cells using a light microscope.
RESULTS
The break of HTM cell membrane was found under the electron microscope. The numbers of microsphere in HTM cells was significantly (P = 0.005) reduced in a dose- and incubation time-dependent manner (F = 3.603, P = 0.046, F = 15.846, P = 0.005, respectively).
CONCLUSIONS
IL-1alpha affects the phagocytic function of HTM cells that is correlated to the concentration and the incubation time.
目的
研究白细胞介素-1α（IL-1α）对体外培养的人小梁网（HTM）细胞吞噬功能的影响。
方法
体外培养并传代HTM细胞。将第3至5代HTM细胞在DMEM/F12培养基中用不同浓度的IL-1α或溶剂处理。每孔加入每细胞1000个0.85微米的微球。通过倒置相差显微镜和电子显微镜观察HTM细胞的形态变化。使用光学显微镜通过计数20个HTM细胞中的微球数量来评估HTM细胞的细胞吞噬功能。
结果
在电子显微镜下发现HTM细胞膜破裂。HTM细胞中的微球数量以剂量和孵育时间依赖性方式显著减少（分别为F = 3.603，P = 0.046，F = 15.846，P = 0.005）。
结论
IL-1α影响HTM细胞的吞噬功能，这与浓度和孵育时间相关。
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