Musmade Prashant, Subramanian G, Srinivasan K K
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal 576104, India.
Anal Chim Acta. 2007 Feb 28;585(1):103-9. doi: 10.1016/j.aca.2006.11.080. Epub 2006 Dec 9.
A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for quantification of aceclofenac in rat plasma. Ibuprofen was used as an internal standard (IS). The present method used protein precipitation for extraction of aceclofenac from rat plasma. Separation was carried out on reversed-phase C(18) column (250 mm x 4.6 mm, 5 micro) and the column effluent was monitored by UV detector at 282 nm. The mobile phase used was methanol-triethylamine (pH 7.0; 0.3% v/v in Milli-Q water) (60:40%, v/v) at a flow rate of 1.0 mL min(-1). This method was linear over the range of 50.0-3500.0 ng mL(-1) with regression coefficient greater than 0.99. The mean recovery of aceclofenac and IS were 84.62+/-3.23 and 89.19+/-1.57%, respectively and the method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of aceclofenac in rats.
建立了一种简单、灵敏的高效液相色谱（HPLC）法，用于定量大鼠血浆中的醋氯芬酸。布洛芬用作内标（IS）。本方法采用蛋白沉淀法从大鼠血浆中提取醋氯芬酸。在反相C(18)柱（250 mm×4.6 mm，5μm）上进行分离，柱流出物通过紫外检测器在282 nm处监测。所用流动相为甲醇-三乙胺（pH 7.0；在超纯水中的体积分数为0.3%，v/v）（60:40%，v/v），流速为1.0 mL min(-1)。该方法在50.0-3500.0 ng mL(-1)范围内呈线性，回归系数大于0.99。醋氯芬酸和内标的平均回收率分别为84.62±3.23%和89.19±1.57%，在研究过程中发现该方法精密、准确且特异。该方法成功应用于大鼠醋氯芬酸的药代动力学研究。
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