一种用于测定新型香豆素类抗炎药物BOL-303225-A的反相高效液相色谱-紫外检测法在大鼠血浆中的建立与验证
Development and validation of an RP-HPLC-UV method for the determination of BOL-303225-A, a new coumarin-based anti-inflammatory drug, in rat plasma.
作者信息
Maltese Adriana, Maugeri Francesco, Ward Keith W, Bucolo Claudio
机构信息
Global Preclinical Development, Bausch and Lomb, Catania, Italy.
出版信息
Biomed Chromatogr. 2007 Apr;21(4):351-5. doi: 10.1002/bmc.757.
A simple and rapid high-performance liquid chromatographic method was developed and validated for the analysis in rat plasma of BOL-303225-A, a new coumarin-based anti-inflammatory drug. Liquid-liquid extraction was used for sample preparation. Chromatographic separation was achieved on a C(18) column using acetonitrile and water containing 1% triethylamine pH 3.5, adjusted with orthophosphoric acid (35.5:64.5 v/v) as mobile phase. The UV detector was set at 324 nm. The method proved to be linear (r(2) > 0.99) and precise (RSD < 7%) over the concentration range 29-940 ng/mL, and was suitable for the support of pharmacokinetic studies in rats.
开发并验证了一种简单快速的高效液相色谱法，用于分析新型香豆素类抗炎药物BOL-303225-A在大鼠血浆中的含量。采用液液萃取法进行样品制备。在C(18)柱上进行色谱分离，以含1%三乙胺（用正磷酸调至pH 3.5）的乙腈和水（35.5:64.5 v/v）为流动相。紫外检测器设定在324 nm。该方法在29 - 940 ng/mL浓度范围内呈线性（r(2) > 0.99）且精密度良好（RSD < 7%），适用于大鼠的药代动力学研究。
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