Khanam Tasneem, Muddashetty Ravi Sondekoppa, Kahvejian Avak, Sonenberg Nahum, Brosius Juergen
Institute of Experimental Pathology, ZMBE, University of Münster, Münster, Germany.
RNA Biol. 2006 Oct;3(4):170-7. doi: 10.4161/rna.3.4.4075. Epub 2006 Oct 26.
Poly(A) binding protein (PABP) binds non-protein-coding BC1 RNA and BC200 RNA, which contain adenosine-rich domains. Two combinations of the four PABP RNA recognition motifs (RRMs), RRMs 1+2 and RRMs 3+4, bind with very strong affinities to various transcripts with long stretches of adenosine residues, whereas RRMs 2+3 bind weakly. While RRMs 1+2 preferentially bind to stretches that contain only adenosines, RRMs 3+4 exhibit relatively high affinities towards sequences that are interspersed with other nucleotides. Binding studies with oligoribonucleotide(A)(65) and oligoribonucleotide(A)(25) showed that the shorter RNA is not an ideal substrate for binding studies to model the interactions with mRNAs, which in general harbor long poly(A) tails.
多聚腺苷酸结合蛋白（PABP）可结合非蛋白质编码的BC1 RNA和BC200 RNA，这两种RNA含有富含腺苷的结构域。四个PABP RNA识别基序（RRMs）中的两种组合，即RRMs 1+2和RRMs 3+4，以非常强的亲和力与具有长链腺苷残基的各种转录本结合，而RRMs 2+3的结合较弱。虽然RRMs 1+2优先结合仅含腺苷的片段，但RRMs 3+4对穿插有其他核苷酸的序列表现出相对较高的亲和力。对寡核糖核苷酸（A）（65）和寡核糖核苷酸（A）（25）的结合研究表明，较短的RNA不是用于结合研究以模拟与通常带有长聚腺苷酸尾巴的mRNA相互作用的理想底物。
