中国的丝虫病传播媒介：致倦库蚊复合组蚊虫的杀虫剂抗性及种群结构

Filariasis vector in China: insecticide resistance and population structure of mosquito Culex pipiens complex.

作者信息

Cui Feng, Tan Yi, Qiao Chuan-Ling

机构信息

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Graduate School, Chinese Academy of Sciences, Beijing 100080, China.

出版信息

Pest Manag Sci. 2007 May;63(5):453-8. doi: 10.1002/ps.1356.

DOI:10.1002/ps.1356

PMID:17387711

Abstract

Seven field populations of mosquito Culex pipiens complex (Diptera: Culicidae) were collected from four provinces of China. The resistance status of larvae to dichlorvos, parathion, chlorpyrifos, fenobucarb (BPMC) and propoxur were determined by bioassays, disclosing that they were more resistant to organophosphate (moderate or low resistance) than to carbamate (low or no significant resistance) insecticides. Starch gel electrophoresis confirmed the presence and distribution of overproduced esterases B1, A2-B2, A8-B8 and A9-B9, the frequencies of which varied according to their regional origins. Electrophoretic polymorphism at four putatively neutral loci (got-1, got-2, pgi and pgm) showed that the overall genetic differentiation found across all populations was significantly large (Fst = 0.28, P < 10(-4)), and genetic exchange was slightly restricted by distance isolation (P = 0.018).

摘要

从中国四个省份采集了七个致倦库蚊复合组（双翅目：蚊科）的野外种群。通过生物测定确定了幼虫对敌敌畏、对硫磷、毒死蜱、仲丁威（BPMC）和残杀威的抗性状况，结果表明它们对有机磷杀虫剂（中度或低度抗性）的抗性高于对氨基甲酸酯类杀虫剂（低度或无显著抗性）。淀粉凝胶电泳证实了过量产生的酯酶B1、A2 - B2、A8 - B8和A9 - B9的存在和分布，其频率因地区来源而异。在四个假定的中性位点（got - 1、got - 2、pgi和pgm）的电泳多态性表明，所有种群间发现的总体遗传分化显著较大（Fst = 0.28，P < 10(-4)），并且遗传交换受到距离隔离的轻微限制（P = 0.018）。