γ射线辐照介质对细菌的作用：与电子传递系统的关系。
The action of gamma-ray-irradiated medium on bacteria: relation to the electron transport system.
作者信息
Frey H E, Pollard E C
机构信息
Biophysics Department, The Pennsylvania State University, University Park, Pennsylvania, USA.
出版信息
Radiat Res. 1968 Oct;36(1):59-67.
Gamma-ray-irradiated medium is found to produce an immediate slowing of respiration in cells of Escherichia coli 15 T-L-. It also introduces a lag in cell division that can be shortened by the presence of methylene blue and potassium cyanide. The lag is absent if the cells are grown anaerobically. These results suggest that the peroxides produced by irradiating medium block the electron transport system. Since cells concentrate peroxides, the loss of this function induced by bombardment was used to deduce the target molecular weight of the enzyme responsible. It is 73,000.
发现经伽马射线辐照的培养基会使大肠杆菌15 T-L-细胞的呼吸立即减缓。它还会导致细胞分裂出现延迟，而亚甲蓝和氰化钾的存在可缩短这种延迟。如果细胞在厌氧条件下生长，则不会出现这种延迟。这些结果表明，辐照培养基产生的过氧化物会阻断电子传输系统。由于细胞会浓缩过氧化物，因此利用轰击诱导的这种功能丧失来推断负责该功能的酶的目标分子量。其为73,000。
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