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过氧化氢酶:其对微生物计数的影响。

Catalase: its effect on microbial enumeration.

作者信息

Martin S E, Flowers R S, Ordal Z J

出版信息

Appl Environ Microbiol. 1976 Nov;32(5):731-4. doi: 10.1128/aem.32.5.731-734.1976.

Abstract

The addition of catalase to the surface of selective medium plates permitted increased enumeration of physically or chemically injured (stressed) microorganisms. Catalase acted by preventing the accumulation of hydrogen peroxide in, or around, injured cells. Heat-injured Staphylococcus aureus cells had decreased catalase activity, and heat-inactivated catalase had no effect on enumeration.

摘要

在选择性培养基平板表面添加过氧化氢酶可增加对物理或化学损伤(应激)微生物的计数。过氧化氢酶通过防止过氧化氢在受损细胞内或周围积累而起作用。热损伤的金黄色葡萄球菌细胞的过氧化氢酶活性降低,热灭活的过氧化氢酶对计数没有影响。

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