Sun Wei, Ding Ya-qin, Teng Wen-hui, Jiao Kui
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Jan;27(1):116-9.
The interaction of acridine orange (AO) and heparin was studied by spectrophotometry. In the pH 2. 0 Britton-Robinson (B-R) buffer solution, AO had a maximum absorption at 478 nm and 492 nm. After the addition of heparin, the absorbance value at 492 nm decreased greatly and a new absorption peak at 453 nm appeared, indicating a strong interaction taking place in the reaction solution. Under the optimal condition, the decrease in absorbance at 492 nm was linear with the concentration of heparin in the range of 3. 0-15.0 mg m L(-1). The m olar absorptivity w as calculated to be E=l. 599X 10(5) L mol(-1) x cm(-1) and the detection limit was 0. 236 mg x L(-1) (3sigma). The method was furthur applied to the detection of heparin sodium injection samples with satisfactory results. The binding number was calculated to be 1 : 3 with molar ratio method.
采用分光光度法研究了吖啶橙（AO）与肝素的相互作用。在pH 2.0的 Britton-Robinson（B-R）缓冲溶液中，AO在478 nm和492 nm处有最大吸收。加入肝素后，492 nm处的吸光度值大幅下降，在453 nm处出现一个新的吸收峰，表明反应溶液中发生了强烈的相互作用。在最佳条件下，492 nm处吸光度的降低与肝素浓度在3.0 - 15.0 mg mL⁻¹范围内呈线性关系。计算得到摩尔吸光系数为E = 1.599×10⁵ L mol⁻¹ cm⁻¹，检测限为0.236 mg L⁻¹（3σ）。该方法进一步应用于肝素钠注射液样品的检测，结果令人满意。用摩尔比法计算结合数为1 : 3。
