采用差示荧光法测定胰岛中的黄素核苷酸。
Assay of flavin nucleotides in pancreatic islets by a differential fluorimetric technique.
作者信息
Brolin S E, Agren A
出版信息
Biochem J. 1977 Apr 1;163(1):159-62. doi: 10.1042/bj1630159.
Abstract
Differences in fluorescence intensity between FMN and FAD at high and low pH values were utilized to determine the concentration of each nucleotide. Amounts down to 1 pmol were measurable. The FMN and FAD contents of pancreatic islets of obese-hyperglycaemic mice (gene symbol ob/ob) were compared with those of the exocrine pancreas, the liver and heart muscle. As expected, the FAD content of each tissue was greater than that of FMN. The contents of both nucleotides were significantly higher in the islets than in the exocrine part of the pancreas, but lower than in the heart muscle and liver. Possible relationships between the flavin content of the islets and their capacity for oxidative phosphorylation are briefly discussed.
摘要
利用黄素单核苷酸（FMN）和黄素腺嘌呤二核苷酸（FAD）在高pH值和低pH值下荧光强度的差异来测定每种核苷酸的浓度。低至1皮摩尔的量都可测量。将肥胖高血糖小鼠（基因符号ob/ob）胰岛中的FMN和FAD含量与外分泌胰腺、肝脏和心肌中的含量进行了比较。正如预期的那样，每个组织的FAD含量都高于FMN。两种核苷酸的含量在胰岛中显著高于胰腺外分泌部分，但低于心肌和肝脏。简要讨论了胰岛黄素含量与其氧化磷酸化能力之间可能的关系。
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