Blanco S, Prat C, Sánchez M D, Ferrer D, Pellicer T, Haba L, Latorre I, Vilaplana C, Ausina V, Domínguez J
Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
Int J Hyg Environ Health. 2008 Mar;211(1-2):168-71. doi: 10.1016/j.ijheh.2007.02.002. Epub 2007 Mar 27.
Despite advances in medium formulations and pretreatment techniques, recovery of Legionella from water samples can still be quite low, difficult and time consuming. The aim of this study was to evaluate the utility of a Legionella urinary antigen enzyme immunoassay (Bartels ELISA, Trinity Biotech, Ireland) for the detection of Legionella in water samples. Reference ATCC Legionella strains were used to spike water samples to a final concentration of 10(4)-10(5)cfu/ml. The lower detection limit of the test for all Legionella pneumophila serogroups was assessed by serial dilutions of spiked water samples. Legionella antigen was detected in all filtered samples except for those spiked with L. bozemanii and L. longbeachae. The lower detection limit for soluble L. pneumophila serogroup 1 antigen was 780cfu/ml. Bartels ELISA could be a useful method for antigen detection in water samples when a high recovery of L. pneumophila is suspected. The test could be used as a rapid screening method for the detection of Legionella in a large number of samples. However, the low sensitivity of the test requires to keep on performing conventional culture for isolation and for further studies on isolated bacteria.
尽管在培养基配方和预处理技术方面取得了进展，但从水样中回收嗜肺军团菌的效率仍然很低，操作困难且耗时。本研究的目的是评估一种嗜肺军团菌尿抗原酶免疫测定法（Bartels ELISA，Trinity Biotech，爱尔兰）用于检测水样中嗜肺军团菌的效用。使用参考ATCC嗜肺军团菌菌株对水样进行加标，使其最终浓度达到10(4)-10(5)cfu/ml。通过对加标水样进行系列稀释来评估该检测方法对所有嗜肺军团菌血清型的最低检测限。在所有过滤后的样品中均检测到了嗜肺军团菌抗原，但用博兹曼嗜肺军团菌和长滩嗜肺军团菌加标的样品除外。可溶性嗜肺军团菌血清1型抗原的最低检测限为780cfu/ml。当怀疑嗜肺军团菌回收率较高时，Bartels ELISA可能是一种检测水样中抗原的有用方法。该检测方法可作为一种快速筛选方法，用于检测大量样品中的嗜肺军团菌。然而，该检测方法的低灵敏度要求继续进行传统培养以进行分离，并对分离出的细菌进行进一步研究。
Zotero 插件