由hSSAO/VAP-1基因稳定转染的A7r5细胞系（A7r5 hSSAO/VAP-1细胞系）的特性分析
Characterization of A7r5 cell line transfected in a stable form by hSSAO/VAP-1 gene (A7r5 hSSAO/VAP-1 cell line).
作者信息
Solé M, Hernandez M, Boada M, Unzeta M
机构信息
Departament de Bioquímica i Biologia Molecular, Facultat de Medicina, Institut de Neurociències, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
出版信息
J Neural Transm (Vienna). 2007;114(6):763-7. doi: 10.1007/s00702-007-0685-9. Epub 2007 Mar 29.
A smooth muscle cell line (A7r5) was stably transfected with the human SSAO/VAP-1 (hSSAO/VAP-1) gene. The expressed protein was located solely in the membrane fraction of the cell. However it was also shown to be released into the cell-culture medium. Both the membrane-bound and released, soluble, forms had SSAO enzyme activity. Although MAO-A is present in wild-type A7r5 cells, it was undetectable in the transfected cells.
一种平滑肌细胞系（A7r5）被稳定转染了人SSAO/VAP-1（hSSAO/VAP-1）基因。所表达的蛋白仅定位于细胞的膜部分。然而，它也被证明会释放到细胞培养基中。膜结合形式和释放的可溶性形式都具有SSAO酶活性。虽然野生型A7r5细胞中存在MAO-A，但在转染细胞中未检测到。
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