Barker Emma V
Department of Otorhinolaryngology, Wessex Deanery, Southampton, UK.
Ann R Coll Surg Engl. 2007 Apr;89(3):197-202. doi: 10.1308/003588407X183256.
Laryngeal transplantation remains an increasingly viable option for patients with irreversible disease or damage to the larynx. Successful organ transplantation relies on minimising surgical, ischaemic and immunological insults. The inherent immunogenicity of an organ is dependent on the amount of immunologically active cells within it. The presence of immunologically active cells within non-transplanted NIH-minipigs was investigated and an in vivo laryngeal transplant model was developed.
Quantitative, multiple-colour immunofluorescence using pig-specific monoclonal antibodies was used to assess the normal immunological architecture and the short-term immunological changes associated with 3 h of cold ischaemia and 8 h of reperfusion in an MHC-matched animal model.
There is a complex immunological architecture within the non-transplanted, healthy pig larynx. In addition, an in vivo laryngeal transplant model was developed that allowed successful perfusion for 8 h post transplantation. There were significant changes in cell numbers within different anatomical subsites of the larynx. However, the biological significance remains debatable in view of the large range of cell numbers both within and between individual animals.
对于患有不可逆性喉疾病或喉部损伤的患者，喉移植仍然是一个越来越可行的选择。成功的器官移植依赖于将手术、缺血和免疫损伤降至最低。器官固有的免疫原性取决于其中免疫活性细胞的数量。研究了未移植的NIH小型猪体内免疫活性细胞的存在情况，并建立了一种体内喉移植模型。
在 MHC 匹配的动物模型中，使用猪特异性单克隆抗体进行定量多色免疫荧光分析，以评估正常免疫结构以及与 3 小时冷缺血和 8 小时再灌注相关的短期免疫变化。
未移植的健康猪喉内存在复杂的免疫结构。此外，还建立了一种体内喉移植模型，该模型使移植后成功灌注 8 小时成为可能。喉部不同解剖亚部位的细胞数量发生了显著变化。然而，鉴于个体动物内部和之间细胞数量的广泛范围，其生物学意义仍存在争议。
