Konno Sensuke
New York Medical College; Department of Urology; Munger Pavilion 4th Floor; Valhalla, NY 10595, USA.
Altern Med Rev. 2007 Mar;12(1):63-8.
Despite the availability of several therapeutic options, a safer and more effective modality is urgently needed for treatment of bladder cancer. Specific immunotherapy is effective, but severe side effects limit its clinical use and underscore the need for unconventional therapies using less toxic substances. Many natural substances are touted for their medicinal aspects and side effect profiles, and some of these have been well characterized for their biological and medicinal properties. Accordingly, the effects on bladder cancer cells in vitro were investigated. Eight commercially available natural products were tested for possible effects on the growth of human bladder cancer T24 cells. This study demonstrated that two mushroom extracts, GD- and PL-fractions, induced a significant (>90 percent) growth reduction in 72 hours, whereas the remaining six products had no effect. Interestingly, non-toxic concentrations of the GD- or PL-fractions, when combined with a non-toxic concentration of vitamin C, became highly cytotoxic, resulting in >90-percent cell death. Thus, vitamin C appears to act synergistically with these fractions to potentiate their bioactivity (cytotoxicity). No other products tested demonstrated such a synergistic potentiation with vitamin C. The present study indicates that GD- and PL-fractions appear to have the most potent cytotoxic effect on human bladder cancer T24 cells. It is thus plausible that these substances could be used, solely or combined with conventional modalities, for the treatment of superficial bladder cancer.
尽管有多种治疗选择，但膀胱癌的治疗迫切需要一种更安全、更有效的方法。特异性免疫疗法是有效的，但严重的副作用限制了其临床应用，并凸显了使用毒性较小的物质进行非常规治疗的必要性。许多天然物质因其药用价值和副作用特征而受到吹捧，其中一些物质的生物学和药用特性已得到充分表征。因此，研究了它们对体外膀胱癌细胞的影响。测试了八种市售天然产物对人膀胱癌T24细胞生长的可能影响。这项研究表明，两种蘑菇提取物，GD组分和PL组分，在72小时内可使细胞生长显著减少（>90%），而其余六种产物则无此作用。有趣的是，GD或PL组分的无毒浓度与无毒浓度的维生素C联合使用时，会变得具有高度细胞毒性，导致>90%的细胞死亡。因此，维生素C似乎与这些组分协同作用，增强了它们的生物活性（细胞毒性）。测试的其他产物均未显示与维生素C有这种协同增强作用。本研究表明，GD和PL组分似乎对人膀胱癌T24细胞具有最有效的细胞毒性作用。因此，这些物质单独或与传统方法联合用于浅表性膀胱癌的治疗是合理的。
