Rajamani Rathinam, Muthuvel Arumugam, Manikandan Sundaramahalingam, Srikumar Ramasundaram, Sheeladevi Rathinasamy
Department of Physiology, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, Tamilnadu, India.
Chem Biol Interact. 2007 May 1;167(3):161-7. doi: 10.1016/j.cbi.2007.01.019. Epub 2007 Feb 12.
DL-alpha-Lipoic acid (LPA) was reported to be effective in reducing free radicals generated by oxidative stress. The protective of effect of LPA on methanol (MeOH) induced free radical changes and oxidative damages in discrete regions of rat brain have been reported in this study. Folate deficient rat (FDD) model was used. The five animal groups (saline control, FDD control, FDD+MeOH, FDD+LPA+MeOH, LPA control) were used. The FDD+MeOH and FDD+LPA+MeOH animals were injected intraperitoneally with methanol (3gm/kg). After 24h, the level of free radical scavengers such as, superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione was estimated in six discrete regions of brain, retina and optic nerve. Level of protein thiol, protein carbonyl and lipid peroxidation was also estimated. Expression of heat shock protein 70 mRNA (hsp70) was studied in the cerebellum and hippocampus by reverse transcriptase PCR. All the samples showed elevation in the level of free radical scavenging enzymes and reduced level of glutathione in the FDD+MeOH group in relation to the other groups. hsp70 expression was more in FDD+MeOH group when compared to FDD+LPA+MeOH group. In conclusion, MeOH exposure leads to increased free radical generation and protein oxidative damages in the rat nervous tissue. Treatment with LPA prevents oxidative damage induced by MeOH exposure.
据报道，DL-α-硫辛酸（LPA）在减少氧化应激产生的自由基方面有效。本研究报道了LPA对甲醇（MeOH）诱导的大鼠脑离散区域自由基变化和氧化损伤的保护作用。使用了叶酸缺乏大鼠（FDD）模型。采用了五个动物组（生理盐水对照组、FDD对照组、FDD+MeOH组、FDD+LPA+MeOH组、LPA对照组）。FDD+MeOH组和FDD+LPA+MeOH组动物腹腔注射甲醇（3克/千克）。24小时后，在脑、视网膜和视神经的六个离散区域评估超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、还原型谷胱甘肽等自由基清除剂的水平。还评估了蛋白质巯基、蛋白质羰基和脂质过氧化水平。通过逆转录聚合酶链反应研究小脑和海马中热休克蛋白70 mRNA（hsp70）的表达。与其他组相比，FDD+MeOH组所有样本的自由基清除酶水平升高，谷胱甘肽水平降低。与FDD+LPA+MeOH组相比，FDD+MeOH组hsp70表达更多。总之，甲醇暴露导致大鼠神经组织中自由基生成增加和蛋白质氧化损伤。LPA治疗可预防甲醇暴露诱导的氧化损伤。
