Hung Shao-Wen, Wang Shu-Ling, Tu Ching-Yu, Tsai Yueh-Chih, Chuang Shih-Te, Shieh Meng-Tong, Liu Pan-Chen, Wang Way-Shyan
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 250, Kuo-Kuang Road, Taichung City 402, Taiwan.
Vet J. 2008 May;176(2):197-204. doi: 10.1016/j.tvjl.2007.02.008. Epub 2007 Mar 30.
The aim of this study was to investigate drug resistance and the genetic relatedness of erythromycin-resistant Streptococcus spp. from different animals and humans in Taiwan. Cumulatively, 248 isolates were collected from 15 animal species and human patients and the susceptibilities of the isolates to six antimicrobial agents including azithromycin (AZI), clarithromycin (CLAR), erythromycin (ERY), spiramycin (SPIR), amoxicillin (AMO), and enrofloxacin (ENRO) were determined by the agar dilution method. The results indicated that resistance among the 248 strains was highest for SPIR, followed by ENRO, CLAR, ERY, AZI, and AMO. The most common resistotypes of the isolates from mammals and aquatic animals were AZI-CLAR-ERY-SPIR (27.5%) and SPIR (55.1%), respectively. The presence of ERY-resistant genes was confirmed by PCR. The erm gene was amplified from 28 isolates (20.6%) by PCR for further investigation. The predominant erm gene in the ERY-resistant isolates was the erm(B) gene. The phylogenetic analysis of the erm(B) gene results indicated that there was a close genetic relationship among all the strains but the genotypic clusters did not show clear segregation of the isolates according to the source or region.
本研究旨在调查台湾不同动物和人类中耐红霉素链球菌属的耐药性及基因相关性。累计从15种动物和人类患者中收集了248株分离株，并采用琼脂稀释法测定了这些分离株对阿奇霉素（AZI）、克拉霉素（CLAR）、红霉素（ERY）、螺旋霉素（SPIR）、阿莫西林（AMO）和恩诺沙星（ENRO）六种抗菌药物的敏感性。结果表明，248株菌株中对SPIR的耐药性最高，其次是ENRO、CLAR、ERY、AZI和AMO。来自哺乳动物和水生动物的分离株最常见的耐药模式分别为AZI-CLAR-ERY-SPIR（27.5%）和SPIR（55.1%）。通过PCR确认了耐ERY基因的存在。通过PCR从28株分离株（20.6%）中扩增出erm基因以进行进一步研究。耐ERY分离株中主要的erm基因是erm(B)基因。erm(B)基因的系统发育分析结果表明，所有菌株之间存在密切的遗传关系，但基因型聚类并未根据来源或地区显示出分离株的明显分离。
