Jackson-Lewis Vernice, Przedborski Serge
Department of Neurology, Columbia University, New York, NY 10032, USA.
Nat Protoc. 2007;2(1):141-51. doi: 10.1038/nprot.2006.342.
This protocol describes our method of producing a reliable mouse model of Parkinson's disease (PD) using the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). We discuss the particulars of the model, provide key references and outline what investigators need to know to develop the MPTP mouse model of PD safely and successfully. Completion of this protocol depends on the regimen of MPTP used and on the actual planned studies, which often range from 7 to 30 d. This protocol calls for implementation of safety measures and for the acquisition of several pieces of equipment, which are a one-time investment worth making if one elects to use this model on a regular basis.
本方案描述了我们使用神经毒素1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）制作可靠的帕金森病（PD）小鼠模型的方法。我们讨论了该模型的细节，提供了关键参考文献，并概述了研究人员为安全、成功地开发MPTP小鼠PD模型需要了解的内容。本方案的完成取决于所使用的MPTP给药方案以及实际计划的研究，这些研究通常持续7至30天。本方案要求实施安全措施并购置几件设备，如果选择定期使用该模型，这些都是值得一次性投入的。
