Rodriguez G Marcela, Gardner Richard, Kaur Navneet, Phanstiel Otto
The Public Health Research Institute at the International Center for Public Health, University of Medicine and Dentistry of New Jersey, 225 Warren Street, Newark, NJ 07103-3535, USA.
Biometals. 2008 Feb;21(1):93-103. doi: 10.1007/s10534-007-9096-5. Epub 2007 Mar 31.
Mycobacterium tuberculosis, the causative agent of human tuberculosis, synthesizes and secretes siderophores in order to compete for iron (an essential micronutrient). Successful iron acquisition allows M. tuberculosis to survive and proliferate under the iron-deficient conditions encountered in the host. To examine structural determinants important for iron siderophore transport in this pathogen, the citrate-based siderophores petrobactin, acinetoferrin and various acinetoferrin homologs were synthesized and used as iron transport probes. Mutant strains of M. tuberculosis deficient in native siderophore synthesis or transport were utilized to better understand the mechanisms involved in iron delivery via the synthetic siderophores. Acinetoferrin and its derivatives, especially those containing a cyclic imide group, were able to deliver iron or gallium into M. tuberculosis which promoted or inhibited, respectively, the growth of this pathogen.
结核分枝杆菌是人类结核病的病原体，它合成并分泌铁载体以竞争铁（一种必需的微量营养素）。成功获取铁使结核分枝杆菌能够在宿主中遇到的缺铁条件下存活和增殖。为了研究该病原体中铁载体运输重要的结构决定因素，合成了基于柠檬酸盐的铁载体杆菌铁载体、醋铁菌素及各种醋铁菌素同系物，并将其用作铁运输探针。利用缺乏天然铁载体合成或运输的结核分枝杆菌突变菌株，以更好地了解通过合成铁载体进行铁传递所涉及的机制。醋铁菌素及其衍生物，特别是那些含有环状酰亚胺基团的衍生物，能够将铁或镓递送至结核分枝杆菌中，分别促进或抑制该病原体的生长。
